A highly selective and fast-response fluorescent probe for visualization of enzymatic H2S production in vitro and in living cells

Lv Wei; Zhentao Zhu; Yanyan Li; Long Yi; Zhen Xi

doi:10.1039/c5cc03707g

A highly selective and fast-response fluorescent probe for visualization of enzymatic H2S production in vitro and in living cells

Chem Commun (Camb). 2015 Jul 4;51(52):10463-6. doi: 10.1039/c5cc03707g.

Authors

Lv Wei¹, Zhentao Zhu, Yanyan Li, Long Yi, Zhen Xi

Affiliation

¹ State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University, Tianjin, 300071, China. zhenxi@nankai.edu.cn.

PMID: 26027437
DOI: 10.1039/c5cc03707g

Abstract

An o-fluorinated-azido-capped rhodamine probe can react with H2S efficiently and selectively to give large off-on fluorescence enhancement. The probe was used to develop an assay for cystathionine β-synthase acitivity and for in situ visualization of endogenously produced H2S in living cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival
Cystathionine beta-Synthase / metabolism*
Fluorescence
Fluorescent Dyes / chemistry*
Fluorescent Dyes / metabolism
HEK293 Cells
Humans
Hydrogen Sulfide / chemistry
Hydrogen Sulfide / metabolism*
Rhodamines / chemistry*
Rhodamines / metabolism

Substances

Fluorescent Dyes
Rhodamines
Cystathionine beta-Synthase
Hydrogen Sulfide