A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

Lihua Lu; Modi Wang; Li-Juan Liu; Chun-Yuen Wong; Chung-Hang Leung; Dik-Lung Ma

doi:10.1039/c5cc02790j

A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

Chem Commun (Camb). 2015 Jun 21;51(49):9953-6. doi: 10.1039/c5cc02790j.

Authors

Lihua Lu¹, Modi Wang, Li-Juan Liu, Chun-Yuen Wong, Chung-Hang Leung, Dik-Lung Ma

Affiliation

¹ Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. edmondma@hkbu.edu.hk.

PMID: 25999030
DOI: 10.1039/c5cc02790j

Abstract

An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL(-1), and the limit of detection for TdT was 0.25 U mL(-1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry
DNA / metabolism
DNA Nucleotidylexotransferase / metabolism*
Enzyme Assays / methods*
Iridium / chemistry*
Luminescent Agents / chemistry*
Luminescent Measurements
Nucleotide Motifs*
Organometallic Compounds / chemistry
Phenanthrolines / chemistry

Substances

Luminescent Agents
Organometallic Compounds
Phenanthrolines
Iridium
DNA
DNA Nucleotidylexotransferase