Developmentally expressed O-acetyl ganglioside GT3 in fetal rat cerebral cortex

Neurosci Lett. 1989 Nov 20;106(1-2):193-8. doi: 10.1016/0304-3940(89)90225-5.

Abstract

Monoclonal antibody M6704, established against the chick neural tube, was shown to recognize a trisialosyl residue, NeuAc alpha 2-8NeuAc alpha 2-8NeuAc alpha 2-3-R of C-series gangliosides. Using this antibody, the developmental changes of C-series gangliosides in fetal rat cerebral cortex have been examined. Two dimensional thin layer chromatography (TLC) enzyme-immunostaining analysis revealed that alkali treatment resulted in a great increase in GT3 that amounted to more than 85% of the total GT3 detected. The alkalilabile form was easily degraded to form GT3 by the action of the receptor-destroying enzyme of influenza C virus, sialate O-acetylesterase, indicating that the antigen was most probably 9-O-acetyl-NeuAc containing GT3. The ganglioside was highly enriched at the 14th gestation day, gradually decreased, and was not detected in adult rat cerebral cortex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism*
  • Animals
  • Antibodies, Monoclonal
  • Cerebral Cortex / embryology
  • Cerebral Cortex / growth & development
  • Cerebral Cortex / metabolism*
  • Embryonic and Fetal Development*
  • Gangliosides / metabolism*
  • Glycosphingolipids / metabolism*
  • Lactosylceramides / metabolism*
  • Rats
  • Rats, Inbred Strains

Substances

  • Antibodies, Monoclonal
  • Gangliosides
  • Glycosphingolipids
  • Lactosylceramides
  • trisialosyllactosylceramide