Determination of Sudan Residues in Sausage by Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography

J Chromatogr Sci. 2015 Sep;53(8):1333-40. doi: 10.1093/chromsci/bmv017. Epub 2015 Apr 1.

Abstract

A method based on matrix solid-phase dispersion and high-performance liquid chromatography was applied to the determination of four Sudan red residues in sausage. The proposed method required only 0.5 g sample. The neutral alumina was used as the dispersant sorbent while n-hexane containing 10% (v/v) acetone was used as the eluting solvent. The recoveries in samples ranged from 76.4 to 111.0% and relative standard deviations were <10.2% with different spiked levels of 0.05-1.50 mg kg(-1). Detection and quantification limits were 5-19 and 18-39 μg kg(-1), respectively, with linear calibration curves extending up to 2.50 mg g(-1). The results demonstrate that the method can be successfully applied with acceptable recoveries to a broad range of target analytes within a diverse range of sausage.

MeSH terms

  • Azo Compounds / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Coloring Agents / analysis*
  • Linear Models
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Solid Phase Extraction / methods*

Substances

  • Azo Compounds
  • Coloring Agents