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EMBO J. 1989 Nov;8(11):3419-28.

Proper expression of myosin genes in transgenic nematodes.

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  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.

Erratum in

  • EMBO J 1989 Dec;8(13):4359.

Abstract

Caenorhabditis elegans has four genes which encode skeletal myosin heavy chain isoforms. We have re-introduced clones of two of these genes, myo-3 and unc-54 at low copy number into the germline of C. elegans. The resulting loci behave as functional copies of the genes by two genetic criteria: (i) they can result in phenotypic rescue of strains carrying inactivating myo-3 or unc-54 mutations, and (ii) their presence in strains with wild-type copies of the endogenous myosin loci has genetic consequences similar to duplicating the endogenous loci. The re-introduced genes function at a level close to that of the endogenous loci. Monoclonal antibodies specific for the different isoforms have been used to localize the expressed proteins. The re-introduced genes express in precisely the same cell types as the endogenous genes, and the myosin products produced assemble into filament structures as in wild-type. Unexpectedly, we have found in the course of this work that very high copy numbers of the unc-54 gene lead to a disruption of muscle structure which may result from overexpression of the protein product.

PMID:
2583105
[PubMed - indexed for MEDLINE]
PMCID:
PMC401492
Free PMC Article
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