LED 590 nm photomodulation reduces UVA-induced metalloproteinase-1 expression via upregulation of antioxidant enzyme catalase

J Dermatol Sci. 2015 May;78(2):125-32. doi: 10.1016/j.jdermsci.2015.02.018. Epub 2015 Mar 5.

Abstract

Background: Light at visible spectrum has been associated with anti-inflammatory and anti-aging effects. Ultraviolet A (UVA) radiation is the most important environmental factor associated with exogenous aging via induction of reactive oxygen species (ROS).

Objective: In this study, we focused on elucidating the molecular mechanisms involved in biological effects associated with 590 nm light delivered from light emitting diode (LED).

Methods: UVA-induced metalloproteinase-1 (MMP-1) expression in dermal fibroblast was used as a model system for investigation.

Results: Pretreating cultured human fibroblasts with 590 nm light attenuated UVA-induced ROS, phosphorylated Jun N-terminal kinases, and MMP-1 expressions in a sequential manner. Pretreatment with potent antioxidant N-acetylcysteine produced similar effect, suggesting enhanced antioxidant capacity induced by 590 nm photomodulation. Further experiments demonstrated that 590 nm photomodulation attenuated UVA-induced ROS and MMP-1 expressions via mitochondrial retrograde signaling that augments the antioxidant enzyme expression in a peroxisome proliferators-activated receptor γ coactivator-1α-dependent manner.

Conclusion: Our results provided possible mechanistic insights explaining the effect of visible light on treating clinical conditions associated with ROS-mediated dysfunctions.

Keywords: 590nm light; Catalase; Peroxisome proliferators-activated receptor γ coactivator-1α; Reactive oxygen species; UVA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcysteine / pharmacology
  • Antioxidants / metabolism
  • Catalase / genetics
  • Catalase / metabolism*
  • Cell Survival / radiation effects
  • Cells, Cultured
  • Fibroblasts / enzymology
  • Fibroblasts / radiation effects
  • Gene Silencing
  • Humans
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Light*
  • Matrix Metalloproteinase 1 / metabolism*
  • Mitochondria / enzymology
  • Mitochondria / radiation effects
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / radiation effects
  • Skin / enzymology*
  • Skin / radiation effects*
  • Skin Aging / radiation effects*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Ultraviolet Rays
  • Up-Regulation / radiation effects

Substances

  • Antioxidants
  • PPARGC1A protein, human
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Reactive Oxygen Species
  • Transcription Factors
  • Catalase
  • JNK Mitogen-Activated Protein Kinases
  • Matrix Metalloproteinase 1
  • Acetylcysteine