Human BK Polyomavirus Plasmid pBKV (34-2) (Dunlop) Contains Mutations Not Found in the Originally Published Sequences

Stian Henriksen; Christian Mittelholzer; Rainer Gosert; Hans H Hirsch; Christine Hanssen Rinaldo

doi:10.1128/genomeA.00046-15

Human BK Polyomavirus Plasmid pBKV (34-2) (Dunlop) Contains Mutations Not Found in the Originally Published Sequences

Genome Announc. 2015 Mar 26;3(2):e00046-15. doi: 10.1128/genomeA.00046-15.

Authors

Stian Henriksen¹, Christian Mittelholzer², Rainer Gosert², Hans H Hirsch, Christine Hanssen Rinaldo³

Affiliations

¹ Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway.
² Transplantation and Clinical Virology, Department Biomedicine (Haus Petersplatz), University of Basel, Basel, Switzerland.
³ christine.rinaldo@unn.no.

Abstract

The plasmid pBKV (34-2) (ATCC 45025) contains the entire BK polyomavirus Dunlop genome. Sequencing revealed 12 point mutations compared to the GenBank sequence, but only 4 point mutations compared to the published sequence. The origin of these differences is unknown, but may impact virological as well as diagnostic research and development.