APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

Chem Commun (Camb). 2015 Mar 18;51(22):4697-700. doi: 10.1039/c5cc00438a.

Abstract

We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL(-1)) and could reliably diagnose malaria in clinical blood samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / metabolism*
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Colorimetry*
  • Erythrocytes / metabolism
  • Erythrocytes / parasitology
  • Humans
  • Lactate Dehydrogenases / blood
  • Lactate Dehydrogenases / isolation & purification*
  • Lactate Dehydrogenases / metabolism
  • Malaria / diagnosis*
  • Malaria / parasitology
  • Malaria / pathology
  • Plasmodium falciparum / enzymology*
  • Rats
  • Sensitivity and Specificity

Substances

  • Aptamers, Nucleotide
  • Biomarkers
  • Lactate Dehydrogenases