Abstract
We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction of the endogenous messenger RNA arrests the extracts in interphase. The addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles. The newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cell Cycle*
-
Cell Nucleus / ultrastructure
-
Embryo, Nonmammalian / cytology*
-
Embryo, Nonmammalian / physiology
-
Female
-
Growth Substances / physiology
-
Interphase
-
Maturation-Promoting Factor
-
Metaphase
-
Mitosis
-
Nuclear Proteins / biosynthesis*
-
Nuclear Proteins / genetics
-
Nuclear Proteins / physiology
-
Proliferating Cell Nuclear Antigen
-
RNA, Messenger / genetics
-
Xenopus
Substances
-
Growth Substances
-
Nuclear Proteins
-
Proliferating Cell Nuclear Antigen
-
RNA, Messenger
-
Maturation-Promoting Factor