Abstract
Mutation Yellow at the agouti locus in mice (AY/a-mice) causes the increase of food intake and development of obesity and type 2 diabetes. In AY/a-females the disturbances of glucose and fat metabolisms occur after puberty. Previously we demonstrated that in AY/a-females regulation of blood glucose levels by estradiol was impaired. To investigate the mechanisms of estradiol regulatory action disturbance we determined the effects of ovariectomy and estradiol treatment on mRNA GLUT4 levels in adipose tissue and muscles in AY/a-females. C57BL/6J females, not carrying the Yellow mutation at the agouti locus (a/a-mice), were used as a control. The data suggest that one of the reasons for violations of estradiol regulation of glucose metabolism in AY/a-females is decreasing GLUT4 gene transcription activity in muscle tissue.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / drug effects
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Adipose Tissue / metabolism*
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Adipose Tissue / physiopathology
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Agouti Signaling Protein / genetics
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Agouti Signaling Protein / metabolism
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Animals
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Blood Glucose / metabolism
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Diabetes Mellitus, Type 2 / genetics
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Diabetes Mellitus, Type 2 / metabolism*
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Diabetes Mellitus, Type 2 / physiopathology
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Estradiol / pharmacology
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Female
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Gene Expression Regulation
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Glucose Transporter Type 4 / genetics*
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Glucose Transporter Type 4 / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Muscle, Skeletal / drug effects
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Muscle, Skeletal / metabolism*
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Muscle, Skeletal / physiopathology
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Obesity / genetics
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Obesity / metabolism*
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Obesity / physiopathology
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Ovariectomy*
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Transcription, Genetic
Substances
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Agouti Signaling Protein
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Blood Glucose
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Glucose Transporter Type 4
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RNA, Messenger
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Slc2a4 protein, mouse
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Estradiol