A selective colorimetric and fluorescent sensor for Al3+ ion and its application to cellular imaging

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5:140:509-15. doi: 10.1016/j.saa.2015.01.015. Epub 2015 Jan 17.

Abstract

A new rhodamine-based fluorescent turn-on chemosensor (L) for selective detection of Al3+ ion has been developed and characterized. The fluorescent chemosensor L was synthesized by the reaction of intermediate (4) with 2,5-bis (4-phenylacyl chloride)-1,3,4-oxadiazole (3). The chemosensor L displays an excellent selective and sensitive response to Al3+ ion over other metal ions, in which the spirocyclic (non-fluorescent) to ring opened amide (fluorescent) process was utilized and a 1:2 stoichiometry for L-Al3+ complex was formed with an association constant of 2.03×10(3) M(-1). Furthermore, chemosensor L can be applied as a fluorescent probe for monitoring Al3+ in living cells by performing cell imaging studies.

Keywords: Aluminum(III) ion; Chemosensor; Naked eye; Rhodamine B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum / analysis*
  • Breast Neoplasms / chemistry
  • Breast Neoplasms / pathology
  • Cations / analysis
  • Cell Line, Tumor
  • Colorimetry / methods
  • Female
  • Fluorescent Dyes / chemistry*
  • Humans
  • Microscopy, Fluorescence / methods
  • Optical Imaging / methods*
  • Rhodamines / chemistry*

Substances

  • Cations
  • Fluorescent Dyes
  • Rhodamines
  • Aluminum