Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent

Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1144-1150. doi: 10.1099/ijs.0.000070. Epub 2015 Jan 20.

Abstract

A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6(T), was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9° N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1-1.5 µm in length and 0.5 µm in width. Strain TB-6(T) grew between 45 and 70 °C (optimum 55-60 °C), 0 and 35 g NaCl l(-1) (optimum 20-30 g l(-1)) and pH 4.5 and 7.5 (optimum pH 5.5-6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6(T) occurred with H2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-d-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H2/CO2 (80 : 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6(T) showed that this organism branched separately from the three most closely related genera, Caminibacter , Nautilia and Lebetimonas , within the family Nautiliaceae . Strain TB-6(T) contained several unique fatty acids in comparison with other members of the family Nautiliaceae . Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae , Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6(T) ( = DSM 27783(T) = JCM 19563(T)).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteria, Anaerobic / classification
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Epsilonproteobacteria / classification*
  • Epsilonproteobacteria / genetics
  • Epsilonproteobacteria / isolation & purification
  • Fatty Acids / chemistry
  • Hydrothermal Vents / microbiology*
  • Molecular Sequence Data
  • Nitrates / metabolism
  • Pacific Ocean
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Nitrates
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 7

Associated data

  • GENBANK/KF648717
  • GENBANK/KM065880