Localization of the active type I DNA topoisomerase gene on human chromosome 20q11.2-13.1, and two pseudogenes on chromosomes 1q23-24 and 22q11.2-13.1

Hum Genet. 1989 Dec;84(1):6-10. doi: 10.1007/BF00210661.

Abstract

Different subfragments of a cDNA coding for DNA topoisomerase I were used as probes to determine the chromosomal localization of topoisomerase I sequences in human cells. Southern blotting of restricted DNA from a panel of rodent-human somatic cell hybrids revealed the localization of the complete gene on chromosome 20 and the presence of two truncated topoisomerase I pseudogene sequences on chromosomes 1 and 22. In situ chromosome hybridization experiments confirmed these results showing the location of the complete gene on band q11.2-13.1 of chromosome 20, and the location of the pseudogene sequences on band q23-24 of chromosome 1 and q11.2-13.1 of chromosome 22.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • Chromosomes, Human, Pair 20*
  • Chromosomes, Human, Pair 22*
  • DNA Probes
  • DNA Topoisomerases, Type I / genetics*
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Genes*
  • HeLa Cells / enzymology
  • Humans
  • Nucleic Acid Hybridization
  • Pseudogenes*

Substances

  • DNA Probes
  • DNA, Neoplasm
  • DNA Topoisomerases, Type I