Poly(L-lactic acid)-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography

J Sep Sci. 2015 Mar;38(5):720-3. doi: 10.1002/jssc.201401243. Epub 2015 Jan 28.

Abstract

Poly(L-lactic acid) is a linear aliphatic thermoplastic polyester that can be produced from renewable resources. A poly(L-lactic acid)-modified silica stationary phase was newly prepared by amide bond reaction between amino groups on aminopropyl silica and carboxylic acid groups at the end of the poly(L-lactic acid) chain. The poly(L-lactic acid)-silica column was characterized in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography with the use of different mobile phase compositions. The poly(L-lactic acid)-silica column was found to work in both modes, and the retention of test compounds depending on acetonitrile content exhibited "U-shaped" curves, which was an indicator of reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography mixed-mode retention behavior. In addition, carbonyl groups included into the poly(L-lactic acid) backbone work as an electron-accepting group toward a polycyclic aromatic hydrocarbon and provide π-π interactions.

Keywords: Hydrophilic interaction chromatography; Mixed-mode chromatography; Poly(L-lactic acid); Reversed-phase chromatography.

MeSH terms

  • Chromatography, High Pressure Liquid / instrumentation*
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Reverse-Phase / instrumentation*
  • Chromatography, Reverse-Phase / methods
  • Hydrogen-Ion Concentration
  • Hydrophobic and Hydrophilic Interactions
  • Lactic Acid / chemistry*
  • Polycyclic Aromatic Hydrocarbons / chemistry
  • Polycyclic Aromatic Hydrocarbons / isolation & purification
  • Polyesters
  • Polymers / chemistry*
  • Silicon Dioxide / chemistry*

Substances

  • Polycyclic Aromatic Hydrocarbons
  • Polyesters
  • Polymers
  • Lactic Acid
  • poly(lactide)
  • Silicon Dioxide