Solvothermal synthesis of ZnO nanoparticles and anti-infection application in vivo

ACS Appl Mater Interfaces. 2015 Jan 21;7(2):1308-17. doi: 10.1021/am507532p. Epub 2015 Jan 9.

Abstract

Zinc oxide nanoparticles (ZnONPs) have been widely studied as the bacteriostatic reagents. However, synthesis of small ZnO nanoparticles with good monodispersion and stability in aqueous solution is still a challenge. Anti-infection research of ZnONPs used as antibacterial agent in vivo is rare. In this paper, a novel, sustainable, and simple method to synthesize ZnO nanoparticles with good monodispersion in aqueous low-temperature conditions and with a small molecule agent is reported. Inhibition zone test and the minimum inhibitory concentration test were performed to examine the antibacterial activity of ZnONPs against bacteria Staphylococcus aureus and Escherichia coli in vitro. For further application in vivo, low cytotoxicity and low acute toxicity in mice of ZnO were demonstrated. Finally, 4 nm ZnONPs combined with poly(vinyl alcohol) gel was used as antibacterial agent in rodent elytritis model, and significant anti-infection effect was proven. In one word, the present research would shed new light on the designing of antibacterial materials like ZnO with promising application in disinfection.

Keywords: ZnO nanoparticles; antibacterial activities; elytritis model; minimum inhibitory concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Anti-Bacterial Agents / chemistry
  • Escherichia coli / drug effects
  • Female
  • Humans
  • Mice
  • Mice, Inbred ICR
  • Microbial Sensitivity Tests
  • Nanoparticles / administration & dosage
  • Nanoparticles / chemistry*
  • Staphylococcus aureus / drug effects
  • Vaginosis, Bacterial / drug therapy*
  • Vaginosis, Bacterial / microbiology
  • Zinc Oxide / administration & dosage*
  • Zinc Oxide / chemistry

Substances

  • Anti-Bacterial Agents
  • Zinc Oxide