Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta

FEBS Lett. 1989 Sep 11;255(1):21-6. doi: 10.1016/0014-5793(89)81053-1.

Abstract

A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11)beta precursor protein consisting of 499 amino acids including an extension peptide of 24 amino acids at the NH2-terminus. The cDNA contains 1247 nucleotides at the 3'-noncoding region including 51 nucleotides of poly A, but lacks the 5'-noncoding region. The deduced amino acid sequence shows 61% similarity to that of bovine P-450(11)beta. Putative binding sites for heme and steroid are highly conserved among steroidogenic P-450s of known structure.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / enzymology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA / biosynthesis*
  • DNA Restriction Enzymes
  • DNA Transposable Elements
  • Humans
  • Male
  • Molecular Sequence Data
  • Rats
  • Rats, Inbred Strains
  • Species Specificity

Substances

  • DNA Transposable Elements
  • DNA
  • Cytochrome P-450 Enzyme System
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X15431