Eur J Biochem. 1989 Sep 1;184(1):181-6.

Effects of the cellular p53 protein on Simian-virus-40-T-antigen-catalyzed DNA unwinding in vitro.

Source

Fakultät für Biologie, Universität Konstanz.

Abstract

It is known that large T antigen, the regulatory protein encoded by Simian virus 40 (SV40), forms tight complexes with the cellular p53 protein in SV40-transformed rodent cells. Using immunoaffinity procedures we have purified large T antigen and, in separate experiments, the cellular p53 protein. The two proteins formed complexes in vitro which bound well to double-stranded DNA fragments although in a sequence-unspecific manner. Free, uncomplexed T antigen readily converted double-stranded DNA into a single-stranded form whereas in-vitro-formed p53-T-antigen complexes were inactive in this reaction. We conclude that one function of p53 in SV40-transformed mouse cells could be the inhibition of the replication initiating activity of T antigen.

PMID:: 2550225; [PubMed - indexed for MEDLINE]

Free full text

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Identification of the p53 protein domain involved in formation of the simian virus 40 large T-antigen-p53 protein complex. [J Virol. 1986]
Identification of the p53 protein domain involved in formation of the simian virus 40 large T-antigen-p53 protein complex.
Tan TH, Wallis J, Levine AJ. J Virol. 1986 Sep; 59(3):574-83.
The murine p53 protein blocks replication of SV40 DNA in vitro by inhibiting the initiation functions of SV40 large T antigen. [Cell. 1989]
The murine p53 protein blocks replication of SV40 DNA in vitro by inhibiting the initiation functions of SV40 large T antigen.
Wang EH, Friedman PN, Prives C. Cell. 1989 May 5; 57(3):379-92.
Analysis of a large-T-antigen variant expressed in simian virus 40-transformed mouse cell line mKS-A. [J Virol. 1989]
Analysis of a large-T-antigen variant expressed in simian virus 40-transformed mouse cell line mKS-A.
Dora S, Schwarz C, Baack M, Graessmann A, Knippers R. J Virol. 1989 Jun; 63(6):2820-8.
Review p53 and transformation by SV40. [Biol Cell. 1986]
Review p53 and transformation by SV40.
O'Reilly DR. Biol Cell. 1986; 57(3):187-96.
Review The biological activities of simian virus 40 large-T antigen and its possible oncogenic effects in humans. [Monaldi Arch Chest Dis. 1998]
Review The biological activities of simian virus 40 large-T antigen and its possible oncogenic effects in humans.
Matker CM, Rizzo P, Pass HI, Di Resta I, Powers A, Mutti L, Kast WM, Carbone M. Monaldi Arch Chest Dis. 1998 Apr; 53(2):193-7.

See reviews... See all...

Cited by 4 PubMed Central articles

Analysis of genomic instability in Li-Fraumeni fibroblasts with germline p53 mutations. [Oncogene. 1996]
Analysis of genomic instability in Li-Fraumeni fibroblasts with germline p53 mutations.
Liu PK, Kraus E, Wu TA, Strong LC, Tainsky MA. Oncogene. 1996 Jun 6; 12(11):2267-78.
Wild-type p53 is not a negative regulator of simian virus 40 DNA replication in infected monkey cells. [J Virol. 1993]
Wild-type p53 is not a negative regulator of simian virus 40 DNA replication in infected monkey cells.
von der Weth A, Deppert W. J Virol. 1993 Feb; 67(2):886-93.
Assembly of nascent DNA into nucleosome structures in simian virus 40 chromosomes by HeLa cell extract. [J Virol. 1990]
Assembly of nascent DNA into nucleosome structures in simian virus 40 chromosomes by HeLa cell extract.
Sugasawa K, Murakami Y, Miyamoto N, Hanaoka F, Ui M. J Virol. 1990 Oct; 64(10):4820-9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Effects of the cellular p53 protein on Simian-virus-40-T-antigen-catalyzed DNA u...
Effects of the cellular p53 protein on Simian-virus-40-T-antigen-catalyzed DNA unwinding in vitro.
Eur J Biochem. 1989 Sep 1 ;184(1):181-6.

PubMed
The heparin-binding (fibroblast) growth factor family of proteins.
The heparin-binding (fibroblast) growth factor family of proteins.
Annu Rev Biochem. 1989 ;58:575-606.

PubMed
Molecular mechanisms of bone resorption by the osteoclast.
Molecular mechanisms of bone resorption by the osteoclast.
Anat Rec. 1989 Jun ;224(2):317-24.

PubMed
Ego defensiveness, open-closed mindedness, and nurses' attitude toward culturall...
Ego defensiveness, open-closed mindedness, and nurses' attitude toward culturally different patients.
Nurs Res. 1979 May-Jun ;28(3):166-72.

PubMed
Morphological and immunohistochemical investigation of non-Hodgkin's lymphoma co...
Morphological and immunohistochemical investigation of non-Hodgkin's lymphoma combined with Hodgkin's disease.
Histopathology. 1989 Jul ;15(1):35-48.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Effects of the cellular p53 protein on Simian-virus-40-T-antigen-catalyzed DNA unwinding in vitro.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 4 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information