Proteomics investigation reveals cell death-associated proteins of basidiomycete fungus Trametes versicolor treated with Ferruginol

J Agric Food Chem. 2015 Jan 14;63(1):85-91. doi: 10.1021/jf504717x.

Abstract

Ferruginol has antifungal activity against wood-rot fungi (basidiomycetes). However, specific research on the antifungal mechanisms of ferruginol is scarce. Two-dimensional gel electrophoresis and fluorescent image analysis were employed to evaluate the differential protein expression of wood-rot fungus Trametes versicolor treated with or without ferruginol. Results from protein identification of tryptic peptides via liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS) analyses revealed 17 protein assignments with differential expression. Downregulation of cytoskeleton β-tubulin 3 indicates that ferruginol has potential to be used as a microtubule-disrupting agent. Downregulation of major facilitator superfamily (MFS)–multiple drug resistance (MDR) transporter and peroxiredoxin TSA1 were observed, suggesting reduction in self-defensive capabilities of T. versicolor. In addition, the proteins involved in polypeptide sorting and DNA repair were also downregulated, while heat shock proteins and autophagy-related protein 7 were upregulated. These observations reveal that such cellular dysfunction and damage caused by ferruginol lead to growth inhibition and autophagic cell death of fungi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abietanes / pharmacology*
  • Cell Death / physiology*
  • Electrophoresis, Gel, Two-Dimensional
  • Fungal Proteins / analysis*
  • Fungal Proteins / genetics
  • Fungicides, Industrial / pharmacology*
  • Gene Expression Regulation, Fungal / drug effects
  • Proteomics*
  • Trametes / drug effects*
  • Trametes / genetics
  • Trametes / physiology
  • Tubulin Modulators

Substances

  • Abietanes
  • Fungal Proteins
  • Fungicides, Industrial
  • Tubulin Modulators
  • ferruginol