Virtual karyotyping reveals greater chromosomal stability in neural cells derived by transdifferentiation than those from stem cells

Uri Weissbein; Uri Ben-David; Nissim Benvenisty

doi:10.1016/j.stem.2014.10.018

Virtual karyotyping reveals greater chromosomal stability in neural cells derived by transdifferentiation than those from stem cells

Cell Stem Cell. 2014 Dec 4;15(6):687-91. doi: 10.1016/j.stem.2014.10.018.

Authors

Uri Weissbein¹, Uri Ben-David², Nissim Benvenisty³

Affiliations

¹ Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
² Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
³ Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel. Electronic address: nissimb@cc.huji.ac.il.

PMID: 25479746
DOI: 10.1016/j.stem.2014.10.018

Abstract

Neural cells can be derived either from pluripotent or adult stem cells via differentiation or by transdifferentiation from other cell types with the aid of tissue regulators. We compared the chromosomal stability of over 500 neural cell samples from human and mouse with virtual karyotyping (e-karyotyping). We detected notable genomic instability in cells derived from pluripotent or adult stem cells, but surprisingly, transdifferentiated cells seemed more chromosomally stable, except if they were reprogrammed using pluripotency factors.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult Stem Cells / physiology*
Animals
Cell Culture Techniques
Cell Differentiation
Cell Transdifferentiation
Cellular Reprogramming
Chromosomal Instability
Chromosome Aberrations / statistics & numerical data*
Computational Biology / methods
Humans
In Vitro Techniques
Karyotyping
Mice
Neurons / physiology*
Transcriptome