Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
EMBO J. 1989 Apr;8(4):997-1004.

Activity of the transposon Tam3 in Antirrhinum and tobacco: possible role of DNA methylation.

Author information

  • 1John Innes Institute, Norwich, UK.

Abstract

The transposon Tam3 from Antirrhinum majus can transpose in a heterologous host (Nicotiana tabacum); thus the element is autonomous, probably encoding the specific information required for its own transposition. In transgenic tobacco Tam3 rapidly becomes methylated at its ends whilst adjacent flanking sequences remain hypomethylated. This methylation may account for our failure to detect Tam3 transposition in the progeny of transgenic tobacco. Treatment with the inhibitor of cytosine methylation, 5 aza-cytosine appeared to induce transposon related activity at a low level. In Antirrhinum methylation also appears to be associated with inactivation of Tam3 copies.

PMID:
2545443
[PubMed - indexed for MEDLINE]
PMCID:
PMC400906
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk