Differences in replication of attenuated and neurovirulent polioviruses in human neuroblastoma cell line SH-SY5Y

J Virol. 1989 May;63(5):2357-60. doi: 10.1128/JVI.63.5.2357-2360.1989.

Abstract

A base change from C to U at position 472 of the 5' noncoding region of the poliovirus genome is known to be a major determinant of attenuation in the P3/Sabin vaccine strain. To determine the biochemical basis for the attenuated phenotype imparted by this mutation, a cell line in which replication of neurovirulent and attenuated viruses could be distinguished was identified. A pair of P3/Sabin-P2/Lansing viral recombinants that differ only at position 472 was used; the viruses replicated equally well in HeLa cells, but the virus with a U at base 472 was attenuated in mice. In the human neuroblastoma cell line SH-SY5Y, recombinants with a U at base 472 replicated to approximately 10-fold-lower titers than did neurovirulent viruses with a C at this position. Analysis of viral RNA and protein synthesis indicated that translation of the attenuated viral RNA was specifically reduced in SH-SY5Y cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • HeLa Cells
  • Humans
  • Mutation
  • Neuroblastoma / microbiology
  • Neurons / microbiology*
  • Poliovirus / growth & development*
  • Poliovirus / pathogenicity*
  • Protein Biosynthesis
  • RNA, Viral / genetics
  • Time Factors
  • Transcription, Genetic
  • Tumor Cells, Cultured
  • Vaccines, Attenuated*
  • Viral Proteins / biosynthesis
  • Virus Replication

Substances

  • RNA, Viral
  • Vaccines, Attenuated
  • Viral Proteins