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J Bacteriol. 1989 Apr;171(4):2222-5.

Translation of merD in Tn21.

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  • 1Department of Microbiology, University of Georgia, Athens 30602.

Abstract

All four sequenced examples of the mercury resistance (mer) operon of gram-negative bacteria have a promoter-distal reading frame, merD, whose removal has little effect on the resistance phenotype and whose translation has not previously been observed. Using merD-lacZ protein fusions, we show that merD is translated. However, Hg(II)-induced merD expression, as measured by beta-galactosidase activity and immunoblotting, is 10- to 15-fold lower than that of fusions to the gene immediately preceding it, merA.

PMID:
2539363
[PubMed - indexed for MEDLINE]
PMCID:
PMC209882
Free PMC Article
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