Green and sensitive supercritical fluid chromatographic-tandem mass spectrometric method for the separation and determination of flutriafol enantiomers in vegetables, fruits, and soil

J Agric Food Chem. 2014 Nov 26;62(47):11457-64. doi: 10.1021/jf504324t. Epub 2014 Nov 17.

Abstract

A green and sensitive chiral analytical method was developed to determine flutriafol enantiomers in vegetables (tomato, cucumber), fruits (apple, grape), and soil by supercritical fluid chromatography-tandem mass spectrometry. The enantioseparation was performed within 3.50 min using Chiralpak IA-3 column with CO2/methanol (88:12, v/v) as the mobile phase at a 2.2 mL/min flow rate. The postcolumn compensation technology provided with 1% formic acid/methanol greatly improved the ionization efficiency of mass spectrometry. Column temperature, auto back pressure regulator pressure, and flow rate of compensation solvent were optimized to 30 °C, 2200 psi, and 0.1 mL/min, respectively. The simple and fast QuEChERS pretreatment method was adopted. Mean recoveries for flutriafol enantiomers were 77.2-98.9% with RSDs ≤ 9.6% in all matrices. The limits of quantification ranged from 0.41 to 1.18 μg/kg. Well-applied to analyze authentic samples, the developed method could act as a versatile strategy for the analysis of flutriafol enantiomers in food and environmental matrices.

Keywords: QuEChERS; SFC-MS/MS; chiral; flutriafol; green.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Supercritical Fluid / methods*
  • Food Contamination / analysis
  • Fruit / chemistry*
  • Green Chemistry Technology
  • Limit of Detection
  • Reproducibility of Results
  • Soil / chemistry*
  • Soil Pollutants / analysis
  • Stereoisomerism
  • Tandem Mass Spectrometry / methods*
  • Triazoles / analysis*
  • Vegetables / chemistry*

Substances

  • Soil
  • Soil Pollutants
  • Triazoles
  • flutriafol