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Cloning of the cDNA and gene for a human D2 dopamine receptor.
Grandy DK,
Marchionni MA,
Makam H,
Stofko RE,
Alfano M,
Frothingham L,
Fischer JB,
Burke-Howie KJ,
Bunzow JR,
Server AC, et al.
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.
PMID: 2532362 [PubMed - indexed for MEDLINE]
PMCID: PMC298581
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Cited by 26 PubMed Central articles
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Dopamine receptor repertoire of human granulosa cells.
Rey-Ares V, Lazarov N, Berg D, Berg U, Kunz L, Mayerhofer A.
Reprod Biol Endocrinol. 2007 Oct 25; 5:40. Epub 2007 Oct 25.
[Reprod Biol Endocrinol. 2007]
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Dopamine transporter cell surface localization facilitated by a direct interaction with the dopamine D2 receptor.
Lee FJ, Pei L, Moszczynska A, Vukusic B, Fletcher PJ, Liu F.
EMBO J. 2007 Apr 18; 26(8):2127-36. Epub 2007 Mar 22.
[EMBO J. 2007]
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Activation of phosphoinositide 3-kinase by D2 receptor prevents apoptosis in dopaminergic cell lines.
Nair VD, Olanow CW, Sealfon SC.
Biochem J. 2003 Jul 1; 373(Pt 1):25-32.
[Biochem J. 2003]
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