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Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.
Chiron Corporation, Emeryville, CA 94608.
A random-primed complementary DNA library was constructed from plasma containing the uncharacterized non-A, non-B hepatitis (NANBH) agent and screened with serum from a patient diagnosed with NANBH. A complementary DNA clone was isolated that was shown to encode an antigen associated specifically with NANBH infections. This clone is not derived from host DNA but from an RNA molecule present in NANBH infections that consists of at least 10,000 nucleotides and that is positive-stranded with respect to the encoded NANBH antigen. These data indicate that this clone is derived from the genome of the NANBH agent and are consistent with the agent being similar to the togaviridae or flaviviridae. This molecular approach should be of great value in the isolation and characterization of other unidentified infectious agents.
PMID: 2523562 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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Specific activation of 2'-5'oligoadenylate synthetase gene promoter by hepatitis C virus-core protein: a potential for developing hepatitis C virus targeting gene therapy.
Wang Y, Mao SS, He QQ, Zi Y, Wen JF, Feng DY.
World J Gastroenterol. 2009 Jul 7; 15(25):3178-82.
[World J Gastroenterol. 2009]
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Signal transduction pathways in liver and the influence of hepatitis C virus infection on their activities.
Dabrowska MM, Panasiuk A, Flisiak R.
World J Gastroenterol. 2009 May 14; 15(18):2184-9.
[World J Gastroenterol. 2009]
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Trans-complementation of an NS2 defect in a late step in hepatitis C virus (HCV) particle assembly and maturation.
Yi M, Ma Y, Yates J, Lemon SM.
PLoS Pathog. 2009 May; 5(5):e1000403. Epub 2009 May 1.
[PLoS Pathog. 2009]
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