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    Science. 1989 Feb 17;243(4893):937-40.

    Point mutational inactivation of the retinoblastoma antioncogene.

    Source

    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.

    Abstract

    The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.

    PMID:
    2521957
    [PubMed - indexed for MEDLINE]

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