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Identification of IL-1 receptors on human monocytes.
E. I. du Pont de Nemours and Co., Medical Products Department, Glenolden, PA 19036.
The expression and functional analysis of IL-1 beta R on human monocytes were investigated. Binding of 125I-IL-1 to human monocytes was found to be specific and saturable. Scatchard plot analysis revealed a single class of receptors with a binding constant of 600 pM and a receptor density of approximately 100 binding sites per cell. At 37 degrees C 54% of the labeled ligand was internalized over 2 h of incubation. Addition of 0.2% sodium azide to the cells reduced ligand internalization to 9% of total bound. Cross-linking studies revealed that the IL-1R in human monocytes had a Mr of 80 kDa. The addition of IL-1 to monocytes caused changes in membrane Ag expression as assessed by flow cytometric analysis. The results of this study identify IL-1 receptors on monocytes and suggest that IL-1 may act as an effector molecule for monocytes by enhancing expression of Ag correlated with cell differentiation and immune function.
PMID: 2521881 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Active interleukin-1 receptor required for maximal progression of acute pancreatitis.
Norman JG, Fink G, Franz M, Guffey J, Carter G, Davison B, Sexton C, Glaccum M.
Ann Surg. 1996 Feb; 223(2):163-9.
[Ann Surg. 1996]
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Two high-affinity interleukin 1 receptors represent separate gene products.
Chizzonite R, Truitt T, Kilian PL, Stern AS, Nunes P, Parker KP, Kaffka KL, Chua AO, Lugg DK, Gubler U.
Proc Natl Acad Sci U S A. 1989 Oct; 86(20):8029-33.
[Proc Natl Acad Sci U S A. 1989]
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Enhanced immunogenicity of a sequence derived from hepatitis B virus surface antigen in a composite peptide that includes the immunostimulatory region from human interleukin 1.
Rao KV, Nayak AR.
Proc Natl Acad Sci U S A. 1990 Jul; 87(14):5519-22.
[Proc Natl Acad Sci U S A. 1990]
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