This paper presents a novel real-time impedance sensing chip for the evaporation detection of small volume solutions. Time sharing detection is performed for multiple sample measurements by a relay switching technique. In contrast to a conventional weight loss approach, the advantage of this proposed impedance sensing system is that it not only merely requires as little as 0.5 mL of test samples, but also provides high sensitivity and fast detection. More importantly, this proposed impedance sensing chip has advantages of a small chip size and easy decomposition for cleaning and reuse.