Green electrochemical sensing platforms: utilizing hydroxyapatite derived from natural fish scales as a novel electrochemical material for the sensitive detection of kidney injury molecule 1 (KIM-1)

Ying Zhang; Wei Zhang; Qing Zhang; Kaiyang Li; Wei Liu; Yong Liu; Craig E Banks

doi:10.1039/c4an00957f

Green electrochemical sensing platforms: utilizing hydroxyapatite derived from natural fish scales as a novel electrochemical material for the sensitive detection of kidney injury molecule 1 (KIM-1)

Analyst. 2014 Nov 7;139(21):5362-6. doi: 10.1039/c4an00957f.

Authors

Ying Zhang¹, Wei Zhang, Qing Zhang, Kaiyang Li, Wei Liu, Yong Liu, Craig E Banks

Affiliation

¹ National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R. China. wliu@hust.edu.cn.

PMID: 25192033
DOI: 10.1039/c4an00957f

Abstract

Urinary KIM-1 is an ideal biomarker for acute kidney injury diagnosis. The proof-of-concept is demonstrated by utilizing the hydroxyapatite derived from natural fish scales as an electrode material, where the sensing of KIM-1 is shown to be possible for the first time with a linear range from 0.01 to 0.20 μg mL(-1) and a detection limit of 0.017 μg mL(-1) under model conditions; proof-of-concept is demonstrated in spiked urine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomarkers / analysis*
Durapatite / chemistry*
Electrochemical Techniques / instrumentation*
Fishes*
Hepatitis A Virus Cellular Receptor 1
Limit of Detection
Membrane Glycoproteins / analysis*
Receptors, Virus / analysis*

Substances

Biomarkers
HAVCR1 protein, human
Hepatitis A Virus Cellular Receptor 1
Membrane Glycoproteins
Receptors, Virus
Durapatite