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Oncol Rep. 2014 Nov;32(5):1923-30. doi: 10.3892/or.2014.3424. Epub 2014 Aug 20.

MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma.

Author information

  • 1Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
  • 2Department of Pathology, Shenzhen Sixth People's Hospital (Nanshan Hospital), Shenzhen, Guangdong 518052, P.R. China.

Abstract

MicroRNAs (miRNAs) are key regulators in cell processes. Emerging evidence has suggested that there is a direct association between miRNAs and cancer. However, the exact regulatory mechanisms of miRNAs in tumorigenesis are poorly understood. In the present study, we showed that miR-145 is able to significantly reduce mRNA and protein expression levels of A disintegrin and metalloproteinase 17 (ADAM17) in liver cancer cells (SMMC-7721, BEL-7402 and Huh-7). Dual luciferase reporter assays confirmed that ADAM17 is a direct target of miR-145. Notably, we found that miR-145 inhibits cell proliferation and growth activity in SMMC-7721 cells. These results demonstrated that it may be exert the function of tumor suppression in a particular link of cancer cell growth. Further studies revealed that the silencing of ADAM17 decreased the proliferation and growth activity of SMMC-7721 cells. Moreover, it reduced the expression of MMP-9. In conclusion, miR-145 inhibits liver cancer cell proliferation by directly targeting ADAM17. Thus, it may become a promising biological target in the treatment strategy of hepatocellular carcinoma.

PMID:
25174729
[PubMed - indexed for MEDLINE]
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