Induction of colitis in mice (T-cell transfer model)

Benno Weigmann

doi:10.1007/978-1-4939-1212-4_14

Induction of colitis in mice (T-cell transfer model)

Methods Mol Biol. 2014:1193:143-51. doi: 10.1007/978-1-4939-1212-4_14.

Author

Benno Weigmann¹

Affiliation

¹ Medical Clinic I, Research-Campus, University of Erlangen-Nuremberg, Hartmannstr. 14, 91052, Erlangen, Germany, benno.weigmann@uk-erlangen.de.

PMID: 25151004
DOI: 10.1007/978-1-4939-1212-4_14

Abstract

Animal model of intestinal inflammation is essential for the understanding of the pathomechanisms of inflammatory bowel diseases like Crohn's disease and ulcerative colitis. Typically, naïve T-cells were transferred into immunocompromised mice and develop gut inflammation because of inappropriate downregulation. Here, we describe the isolation, purification, and transfer of CD4(+)CD25(-) T-cells into recipients to obtain an experimental colitis model for the analysis of pathogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adoptive Transfer / adverse effects
Animals
CD4-Positive T-Lymphocytes / microbiology
CD4-Positive T-Lymphocytes / transplantation
Colitis / immunology*
Disease Models, Animal*
Female
Homeodomain Proteins / genetics
Interleukin-2 Receptor alpha Subunit / metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, SCID
Mice, Transgenic
T-Lymphocytes / metabolism
T-Lymphocytes / transplantation*

Substances

Homeodomain Proteins
Interleukin-2 Receptor alpha Subunit
RAG-1 protein