Identification of molecular target of diallyl trisulfide in leukemic cells

Shun Suda; Kazunari Watanabe; Yuki Tanaka; Kazuhiro Watanabe; Rie Tanaka; Jun Ogihara; Toyohiko Ariga; Tomomi Hosono-Fukao; Takashi Hosono; Taiichiro Seki

doi:10.1080/09168451.2014.921563

Identification of molecular target of diallyl trisulfide in leukemic cells

Biosci Biotechnol Biochem. 2014;78(8):1415-7. doi: 10.1080/09168451.2014.921563. Epub 2014 Jun 12.

Authors

Shun Suda¹, Kazunari Watanabe, Yuki Tanaka, Kazuhiro Watanabe, Rie Tanaka, Jun Ogihara, Toyohiko Ariga, Tomomi Hosono-Fukao, Takashi Hosono, Taiichiro Seki

Affiliation

¹ a Department of Chemistry and Life Science , College of Bioresource Sciences, Nihon University , Fujisawa , Japan.

PMID: 25130746
DOI: 10.1080/09168451.2014.921563

Abstract

To identify the molecular target of diallyl trisulfide (DATS) in human leukemic cell line U937, we examined modification of thiol group(s) of cellular proteins by the redox 2D PAGE. A unique protein spot appeared by DATS treatment was identified to be heat shock protein 27 (HSP27). Hsp27 is suggested to be one of the molecular target of DATS in U937.

Keywords: U937; diagonal gel electrophoresis; diallyl trisulfide; garlic; heat shock protein 27 (HSP27).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allyl Compounds / pharmacology*
Allyl Compounds / therapeutic use
Antineoplastic Agents / pharmacology*
Antineoplastic Agents / therapeutic use
Apoptosis / drug effects
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Humans
Leukemia / drug therapy
Leukemia / pathology*
Molecular Targeted Therapy*
Sulfides / pharmacology*
Sulfides / therapeutic use

Substances

Allyl Compounds
Antineoplastic Agents
Sulfides
diallyl trisulfide