The excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36

Carbohydr Polym. 2014 Nov 4:112:16-23. doi: 10.1016/j.carbpol.2014.05.068. Epub 2014 Jun 2.

Abstract

Previous study has verified the polysaccharide from the fruiting body of Pleurotus eryngii (PEPE) is capable of decreasing the lipid content in both of cell-line and mouse model. However, little is known about underlying mechanisms and whether this bioactive polysaccharide exists in submerged culture. Here, we verified the excreted polysaccharides EP and EP-1 from submersion culture of P. eryngii have the remarkable inhibitory effects on lipid accumulation in macrophage-derived foam cells. Structure analysis indicates EP-1 consists of D-types of glucose, galactose and mannose with the main β(1 → 3)-glucan glycosidic linkage branched at O-6 by α-D-glucose while EP digested by β-1,3-glucanase fails to decrease the lipid accumulation, suggesting that the special structure is essential for its function. Expression analysis suggests that EP is able to cause the down-regulation of the scavenger receptor-CD36 on both transcription and protein levels. Most importantly, EP can be obtained by fermentation in a mass-production.

Keywords: Excreted polysaccharide; Lipid accumulation; Pleurotus eryngii; Scavenger receptor; Submerged culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD36 Antigens / metabolism*
  • Carbohydrate Conformation
  • Cells, Cultured
  • Down-Regulation / drug effects
  • Foam Cells / drug effects*
  • Foam Cells / metabolism
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / isolation & purification
  • Fungal Polysaccharides / pharmacology*
  • Galactose / analysis
  • Glucose / analysis
  • Lipid Metabolism / drug effects
  • Lipoproteins, LDL / metabolism
  • Macrophages / drug effects
  • Magnetic Resonance Spectroscopy
  • Mannose / analysis
  • Mice
  • Molecular Weight
  • Pleurotus / chemistry*
  • Pleurotus / metabolism
  • Structure-Activity Relationship

Substances

  • CD36 Antigens
  • Fungal Polysaccharides
  • Lipoproteins, LDL
  • oxidized low density lipoprotein
  • Glucose
  • Mannose
  • Galactose