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Vet Immunol Immunopathol. 2014 Sep 15;161(1-2):77-89. doi: 10.1016/j.vetimm.2014.07.004. Epub 2014 Jul 18.

Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum.

Author information

  • 1Moredun Research Institute, Pentlands Science Park, Bush Loan, EH26 0PZ Penicuik, United Kingdom; Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Balcarce, CC276, 7620, Argentina.
  • 2Moredun Research Institute, Pentlands Science Park, Bush Loan, EH26 0PZ Penicuik, United Kingdom.
  • 3Moredun Research Institute, Pentlands Science Park, Bush Loan, EH26 0PZ Penicuik, United Kingdom; Instituto de Ganadería de Montaña (CSIC-ULE), León 24346, Spain.
  • 4Biomathematics & Statistics Scotland (BioSS), EH9 3JZ Edinburgh, United Kingdom.
  • 5Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, EH25 9RG Edinburgh, United Kingdom.
  • 6Moredun Research Institute, Pentlands Science Park, Bush Loan, EH26 0PZ Penicuik, United Kingdom. Electronic address: francesca.chianini@moredun.ac.uk.

Abstract

Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal-foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Cattle; Cytokines; Neospora caninum; Placenta

PMID:
25091332
[PubMed - indexed for MEDLINE]
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