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Arch Oral Biol. 2014 Nov;59(11):1146-54. doi: 10.1016/j.archoralbio.2014.07.001. Epub 2014 Jul 15.

Platelet-rich plasma enhanced umbilical cord mesenchymal stem cells-based bone tissue regeneration.

Author information

  • 1School of Stomatology, Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, PR China. Electronic address: wenyong@sdu.edu.cn.
  • 2Qilu Hospital, Shandong University, Jinan, PR China.
  • 3Jinan Stomatologic Hospital, Jinan, PR China.
  • 4School of Stomatology, Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, PR China.
  • 5School of Stomatology, Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, PR China. Electronic address: yangps@sdu.edu.cn.
  • 6School of Stomatology, Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, PR China. Electronic address: xinxu@sdu.edu.cn.

Abstract

OBJECTIVES:

To evaluate the effects of platelet-rich plasma (PRP) on the proliferation and differentiation of umbilical cord mesenchymal stem cells (UC-MSCs) and explore the possibility that PRP combined with UC-MSCs may be useful for bone tissue regeneration in vivo.

METHODS:

The proliferation potential of UC-MSCs was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The pluripotent differentiation capacity and alkaline phosphatase (ALP) expression were further determined by ALP staining. The expression of osteoblast-associated genes was evaluated by real-time PCR. In addition, rat critical-sized calvarial defects were examined to evaluate bone regeneration in vivo.

RESULTS:

PRP enhanced UC-MSC proliferation, and 10% PRP caused the strongest ALP and Alizarin red staining. At 7 days, the expression levels of ALP, Collagen 1 (COL-1) and Runt-related transcription factor 2 (RUNX2) in the PRP group were higher than those in the FBS group. Newly regenerated bone was observed in the defect areas, and PRP combined with UC-MSCs can accelerate bone regeneration at an early stage.

CONCLUSIONS:

Our current data suggest that UC-MSCs may be utilized in alternative stem cell-based approaches for the reconstruction and regeneration of bone defects, and PRP combined with UC-MSCs can enhance bone regeneration in vivo.

Copyright © 2014 Elsevier Ltd. All rights reserved.

KEYWORDS:

Bone regeneration; Platelet-rich plasma (PRP); Tissue engineering; Umbilical cord mesenchymal stem cells (UC-MSCs)

PMID:
25086868
[PubMed - in process]
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