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Virology. 2014 Sep;464-465:196-205. doi: 10.1016/j.virol.2014.06.040. Epub 2014 Aug 1.

Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells.

Author information

  • 1Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, 200240, China.
  • 2Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The Third People's Hospital of Chongqing, 400014, China.
  • 3Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
  • 4Bornavirus Research Group affiliated to the Free University of Berlin, Berlin, Germany.
  • 5Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
  • 6Department of Rehabilitative Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
  • 7Jingjie PTM BioLab (Hangzhou) Co. Ltd, Hangzhou, 310018, China.
  • 8Advanced Institute of Translational Medicine, Tongji University, Shanghai, 200092, China.
  • 9Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China. Electronic address: xiepeng@cqmu.edu.cn.

Abstract

BACKGROUND:

Borna disease virus (BDV) replicates in the nucleus and establishes persistent infections in mammalian hosts. A human BDV strain was used to address the first time, how BDV infection impacts the proteome and histone lysine acetylation (Kac) of human oligodendroglial (OL) cells, thus allowing a better understanding of infection-driven pathophysiology in vitro.

METHODS:

Proteome and histone lysine acetylation were profiled through stable isotope labeling for cell culture (SILAC)-based quantitative proteomics. The quantifiable proteome was annotated using bioinformatics. Histone acetylation changes were validated by biochemistry assays.

RESULTS:

Post BDV infection, 4383 quantifiable differential proteins were identified and functionally annotated to metabolism pathways, immune response, DNA replication, DNA repair, and transcriptional regulation. Sixteen of the thirty identified Kac sites in core histones presented altered acetylation levels post infection.

CONCLUSIONS:

BDV infection using a human strain impacted the whole proteome and histone lysine acetylation in OL cells.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Bioinformatics-assisted analysis; Borna disease virus; Histone; Human BDV; Lysine acetylation; Oligodendroglia cells; Proteomic

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