Simultaneous determination of galanthamine and lycorine in Lycoris radiata by a capillary electrophoresis with an electrochemiluminescence method

J Sep Sci. 2014 Oct;37(20):3007-12. doi: 10.1002/jssc.201400639. Epub 2014 Sep 25.

Abstract

A novel capillary electrophoresis with electrochemiluminescence determination method was developed for the determination of two alkaloids based on the electrochemiluminescence signal enhancement effect of the tertiary amine group on tris(2,2'-bipyridyl)ruthenium(II). A linear relationship between the electrochemiluminescence peak area and concentrations of galanthamine and lycorine in the range of 0.07 ∼ 17 μg/mL and 0.07 ∼ 18 μg/mL was obtained and the detection limit was 0.008 and 0.002 μg/mL, respectively. The method is selective, simple, and convenient. It had been successfully applied to the analysis of galanthamine and lycorine in Lycoris radiata samples purchased from a local market.

Keywords: Capillary electrophoresis; Electrochemiluminescence; Galanthamine; Lycorine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amaryllidaceae Alkaloids / analysis*
  • Buffers
  • Electrophoresis, Capillary / methods*
  • Galantamine / analysis*
  • Hydrogen-Ion Concentration
  • Luminescence
  • Lycoris / chemistry*
  • Phenanthridines / analysis*

Substances

  • Amaryllidaceae Alkaloids
  • Buffers
  • Phenanthridines
  • Galantamine
  • lycorine