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Int J Med Sci. 2014 Jul 2;11(9):948-54. doi: 10.7150/ijms.9137. eCollection 2014.

Lycopene treatment of prostate cancer cell lines inhibits adhesion and migration properties of the cells.

Author information

  • 1School of Pharmacy and Biomedical Sciences, IBBS, University of Portsmouth, Portsmouth, UK.

Abstract

BACKGROUND:

Consumption of lycopene through tomato products has been suggested to reduce the risk of prostate cancer. Cellular adhesion and migration are important features of cancer progression and therefore a potential target for cancer interception. In the present study we have examined the in vitro effect of lycopene on these processes.

METHODS:

Prostate cancer cell lines PC3, DU145 and immortalised normal prostate cell line PNT-2 were used. The adhesion assay consisted of seeding pre-treated cells onto Matrigel™, gently removing non-adherent cells and quantitating the adherent fraction using WST-1. Migratory potential was assessed using ibidi™ migration chamber inserts, in which a cell-free zone between two confluent areas was allowed to populate over time and the migration measured.

RESULTS:

24 hour incubation of prostate cell lines with 1.15µmol/l lycopene showed a 40% reduction of cellular motility in case of PC3 cells, 58% in DU145 cells and no effect was observed for PNT2 cells. A dose related inhibition of cell adhesion to a basement membrane in the form of Matrigel™ was observed in all three cell lines and it reached statistical significance for PC3 and PNT2 cells at lycopene concentrations ≥1.15µmol/l. However, in case of DU145, only a concentration of 2.3µmol/l showed a significant reduction.

CONCLUSION:

This in vitro investigation indicates that lycopene can influence the cell adhesion and migration properties of cancer cells at a dose which is arguably achievable in patients. The results of our study expand our understanding of a chemo preventive role of lycopene in prostate cancer.

KEYWORDS:

cell adhesion; lycopene; prostate cancer

PMID:
25076850
[PubMed - in process]
PMCID:
PMC4113588
Free PMC Article
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