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Nat Methods. 2014 Aug;11(8):801-7. doi: 10.1038/nmeth.3027.

Deep mutational scanning: a new style of protein science.

Author information

  • 1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
  • 21] Department of Genome Sciences, University of Washington, Seattle, Washington, USA. [2] Department of Medicine, University of Washington, Seattle, Washington, USA. [3] Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA.

Abstract

Mutagenesis provides insight into proteins, but only recently have assays that couple genotype to phenotype been used to assess the activities of as many as 1 million mutant versions of a protein in a single experiment. This approach-'deep mutational scanning'-yields large-scale data sets that can reveal intrinsic protein properties, protein behavior within cells and the consequences of human genetic variation. Deep mutational scanning is transforming the study of proteins, but many challenges must be tackled for it to fulfill its promise.

PMID:
25075907
[PubMed - indexed for MEDLINE]
PMCID:
PMC4410700
Free PMC Article
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