In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy

Dimitrios Kapsokalyvas; Riccardo Cicchi; Nicola Bruscino; Domenico Alfieri; Francesca Prignano; Daniela Massi; Torello Lotti; Francesco S Pavone

doi:10.1364/BOE.5.002405

In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy

Biomed Opt Express. 2014 Jun 24;5(7):2405-19. doi: 10.1364/BOE.5.002405. eCollection 2014 Jul 1.

Authors

Dimitrios Kapsokalyvas¹, Riccardo Cicchi², Nicola Bruscino³, Domenico Alfieri⁴, Francesca Prignano³, Daniela Massi⁵, Torello Lotti⁶, Francesco S Pavone⁷

Affiliations

¹ European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy.
² European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy ; National Institute of Optics, National Research Council (INO-CNR), 50125, Florence, Italy.
³ Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, 50129, Florence, Italy.
⁴ Light4tech Firenze srl, 50018, Scandicci, Italy.
⁵ Department of Surgery and Translational Medicine, University of Florence, 50134, Florence, Italy.
⁶ Department of Dermatology and Venereology, University Guglielmo Marconi, 00193, Rome, Italy.
⁷ European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy ; National Institute of Optics, National Research Council (INO-CNR), 50125, Florence, Italy ; Department of Physics, University of Florence, 50019, Sesto Fiorentino, Italy.

Abstract

Psoriasis is a skin autoimmune disease characterized by hyperkeratosis, hyperproliferation of the epidermis and dilatation of dermal papillary blood vessels. Healthy skin (5 volunteers) and psoriatic lesions (3 patients) were visualized in vivo, with high contrast and resolution, with a Polarization Multispectral Dermoscope and a Multiphoton Microscope. Psoriatic features were identified and quantified. The effective diameter of the superficial blood vessels was measured at 35.2 ± 7.2 μm and the elongated dermal papillae had an effective diameter of 64.2 ± 22.6 μm. The methodologies developed could be employed for quantitative diagnostic purposes and furthermore serve as a monitoring method of the effect of personalized treatments.

Keywords: (100.2980) Image enhancement; (170.1870) Dermatology; (170.6510) Spectroscopy, tissue diagnostics; (170.6900) Three-dimensional microscopy; (180.4315) Nonlinear microscopy.