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Genome Biol Evol. 2014 Aug;6(8):2096-110. doi: 10.1093/gbe/evu160.

Genome evolution and plasticity of Serratia marcescens, an important multidrug-resistant nosocomial pathogen.

Author information

  • 1Interdisciplinary Research Organization, University of Miyazaki, JapanPresent address: Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Japan.
  • 2Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, JapanPresent address: Kashima ONC QC, Oncology DCU, Eisai Demand Chain Systems, Eisai Co., Ltd., Ibaraki, Japan.
  • 3Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université, Marseille, FranceINSERM, U1104, Marseille, FranceCNRS, UMR7280, Marseille, FrancePresent address: CIIL-Inserm U1019, Institut Pasteur de Lille, Lille, France.
  • 4Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Japan.
  • 5Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, JapanDepartment of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Japan.
  • 6Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Japan.
  • 7Earth-Life Science Institute, Tokyo Institute of Technology, Kanagawa, Japan.
  • 8Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan.
  • 9Pathogen Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
  • 10Division of Molecular Microbiology, College of Life Sciences, University of Dundee, United Kingdom.
  • 11Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, Japan.
  • 12Pathogen Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United KingdomDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom nrt@sanger.ac.uk thayash@med.miyazaki-u.ac.jp.
  • 13Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université, Marseille, FranceINSERM, U1104, Marseille, FranceCNRS, UMR7280, Marseille, France.
  • 14Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, JapanDepartment of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Japan nrt@sanger.ac.uk thayash@med.miyazaki-u.ac.jp.

Abstract

Serratia marcescens is an important nosocomial pathogen that can cause an array of infections, most notably of the urinary tract and bloodstream. Naturally, it is found in many environmental niches, and is capable of infecting plants and animals. The emergence and spread of multidrug-resistant strains producing extended-spectrum or metallo beta-lactamases now pose a threat to public health worldwide. Here we report the complete genome sequences of two carefully selected S. marcescens strains, a multidrug-resistant clinical isolate (strain SM39) and an insect isolate (strain Db11). Our comparative analyses reveal the core genome of S. marcescens and define the potential metabolic capacity, virulence, and multidrug resistance of this species. We show a remarkable intraspecies genetic diversity, both at the sequence level and with regards genome flexibility, which may reflect the diversity of niches inhabited by members of this species. A broader analysis with other Serratia species identifies a set of approximately 3,000 genes that characterize the genus. Within this apparent genetic diversity, we identified many genes implicated in the high virulence potential and antibiotic resistance of SM39, including the metallo beta-lactamase and multiple other drug resistance determinants carried on plasmid pSMC1. We further show that pSMC1 is most closely related to plasmids circulating in Pseudomonas species. Our data will provide a valuable basis for future studies on S. marcescens and new insights into the genetic mechanisms that underlie the emergence of pathogens highly resistant to multiple antimicrobial agents.

© The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

KEYWORDS:

Serratia marcescens; genome plasticity; multidrug resistance; virulence

PMID:
25070509
[PubMed - indexed for MEDLINE]
PMCID:
PMC4231636
Free PMC Article
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