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Life Sci. 2014 Aug 28;111(1-2):42-6. doi: 10.1016/j.lfs.2014.07.008. Epub 2014 Jul 24.

Endogenous α-crystallin inhibits expression of caspase-3 induced by hypoxia in retinal neurons.

Author information

  • 1Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; Key Lab of Visual Damage and Regeneration & Restoration of Chongqing 400038, China.
  • 2Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; Key Lab of Visual Damage and Regeneration & Restoration of Chongqing 400038, China; The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400030, China.
  • 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
  • 4Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; Key Lab of Visual Damage and Regeneration & Restoration of Chongqing 400038, China. Electronic address: wangyieye@aliyun.com.

Abstract

AIM:

To investigate the expression of endogenous, hypoxic stress-induced α-crystallin and caspase-3 in rat retinal neurons in vitro.

MAIN METHODS:

Retinal neurons were cultured from Long-Evans rats. The expression of endogenous α-crystallin was analyzed by immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). Furthermore, hypoxic exposure was performed in cultured cells, and the expression of endogenous α-crystallin and caspase-3 was assayed by Western blotting.

KEY FINDINGS:

Positive α-crystallin staining was observed in cultured retinal neurons, and expression of endogenous α-crystallin mRNA peaked 3-5d after inoculation (P<0.05). Moreover, endogenous, hypoxic stress-induced α-crystallin expression increased gradually, peaking 6h after hypoxia. The expression was more abundant compared to the control (P<0. 01) and was associated with a decrease in caspase-3 expression (P<0. 05).

SIGNIFICANCE:

The present study demonstrates that the expression of endogenous α-crystallin in retinal neurons, especially over-expression induced by hypoxic stress, results in the down regulation of caspase-3. The data suggest that endogenous α-crystallin may act as an endogenous neuroprotective factor in retinal neurons.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Caspase-3; Endogenous α-crystallin; Hypoxia; Retinal neurons

[PubMed - indexed for MEDLINE]
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