Format

Send to:

Choose Destination
See comment in PubMed Commons below
Psychiatry Res. 2014 Nov 30;219(3):674-9. doi: 10.1016/j.psychres.2014.05.034. Epub 2014 Jun 5.

Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: relevance to neuropsychiatric disorders.

Author information

  • 1Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil.
  • 2Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
  • 3Department of Biophysics and Investigation Center for Gene Therapy, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
  • 4Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Pharmacology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
  • 5Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
  • 6Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
  • 7Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. Electronic address: sinbelangero@gmail.com.

Abstract

The spontaneously hypertensive rat (SHR) strain was shown to be a useful animal model to study several behavioral, pathophysiological and pharmacological aspects of schizophrenia and attention-deficit/hyperactivity disorder. To further understand the genetic underpinnings of this model, our primary goal in this study was to compare the gene expression profile of neurotransmitter receptors and regulators in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) of SHR and Wistar rats (control group). In addition, we investigated DNA methylation pattern of promoter region of the genes differentially expressed. We performed gene expression analysis using a PCRarray technology, which simultaneously measures the expression of 84 genes related to neurotransmission. Four genes were significantly downregulated in the PFC of SHR compared to Wistar rats (Gad2, Chrnb4, Slc5a7, and Qrfpr) and none in nucleus accumbens. Gad2 and Qrfpr have CpG islands in their promoter region. For both, the promoter region was hypomethylated in SHR group, and probably this mechanism is not related with the downregulation of these genes. In summary, we identified genes that are downregulated in the PFC of SHR, and might be related to the behavioral abnormalities exhibited by this strain.

Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

KEYWORDS:

Chrnb4; DNA methylation; Gad2; Gene expression; Qrfpr; Slc5a7; Spontaneously hypertensive rats

PMID:
25041985
[PubMed - in process]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk