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J Biosci Bioeng. 2014 Dec;118(6):728-31. doi: 10.1016/j.jbiosc.2014.05.022. Epub 2014 Jul 12.

Stimulated production of steroids in Inonotus obliquus by host factors from birch.

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  • 1School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
  • 2Jiangsu Shenhua Pharmaceutical Co., Ltd, 188 Shenhua Road, Huai'an, Jiangsu 211600, People's Republic of China.
  • 3School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China. Electronic address:


Steroids was considered as one of the bioactive components in Inonotus obliquus, while this kind of secondary metabolites are less accumulated in cultured mycelia. In this study, effect of extracts from bark and core of host-related species, birch (Betula platyphylla Suk.), on steroid production of I. obliquus in submerged culture were evaluated. The results showed that all dosages (0.01 and 0.1 g/L) of aqueous extracts and methanol extracts from birch bark and birch core possessed significantly stimulatory effect on steroid production of I. obliquus (P < 0.05). Among the eight extracts, the aqueous extract (0.01 g/L) from birch bark gave the highest steroid production (225.5 ± 8.7 mg/L), which is 97.3% higher than that of the control group. The aqueous extract (0.01 and 0.1 g/L) from birch bark could simultaneously stimulated mycelial growth and steroid content, while the methanol extract from birch bark only elevated the steroid content. High performance liquid chromatography analysis showed that productions of betulin, ergosterol, cholesterol, lanosterol, stigmasterol, and sitosterol in I. obliquus simultaneously increased in the presence of aqueous extract and methanol extract from birch bark. The results presented herein indicate that extracts from birch bark could act as an inducer for steroid biosynthesis of I. obliquus.

Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.


Betula platyphylla; Birch; Inonotus obliquus; Steroids; Submerged fermentation

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