The histone variant H2A.W defines heterochromatin and promotes chromatin condensation in Arabidopsis

Cell. 2014 Jul 3;158(1):98-109. doi: 10.1016/j.cell.2014.06.006.

Abstract

Histone variants play crucial roles in gene expression, genome integrity, and chromosome segregation. We report that the four H2A variants in Arabidopsis define different genomic features, contributing to overall genomic organization. The histone variant H2A.W marks heterochromatin specifically and acts in synergy with heterochromatic marks H3K9me2 and DNA methylation to maintain transposon silencing. In vitro, H2A.W enhances chromatin condensation by promoting fiber-to-fiber interactions via its conserved C-terminal motif. In vivo, H2A.W is required for heterochromatin condensation, demonstrating that H2A.W plays critical roles in heterochromatin organization. Similarities in conserved motifs between H2A.W and another H2A variant in metazoans suggest that plants and animals share common mechanisms for heterochromatin condensation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Chromatin Assembly and Disassembly*
  • DNA Methylation
  • DNA Transposable Elements
  • Genome-Wide Association Study
  • Heterochromatin / metabolism*
  • Histones / chemistry
  • Histones / genetics
  • Histones / metabolism*
  • Molecular Sequence Data
  • Sequence Alignment

Substances

  • Arabidopsis Proteins
  • DNA Transposable Elements
  • Heterochromatin
  • Histones

Associated data

  • GEO/GSE50942