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Biomed Res Int. 2014;2014:519098. doi: 10.1155/2014/519098. Epub 2014 Jun 5.

In vitro antifungal evaluation of seven different disinfectants on acrylic resins.

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  • 1Department of Prosthodontics, Faculty of Dentistry, Gazi University, Emek, 06510 Ankara, Turkey.
  • 2Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Gazi University, 06510 Ankara, Turkey.
  • 3Department of Microbiology, Faculty of Dentistry, Gazi University, 06510 Ankara, Turkey.



The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials.


Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups (n = 10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions of Candida albicans ATCC#90028 and Candida albicans oral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn's test were used for multiple comparisons. Mann Whitney U test was used to compare the surface roughness.


Statistically significant difference (P < 0.05) was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins.


This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.

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