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Peptides. 2014 Sep;59:1-8. doi: 10.1016/j.peptides.2014.06.013. Epub 2014 Jun 30.

Sequence analysis and feeding responses evoked by the large molecular form of gastrin releasing peptide (GRP) in the rat GRP-29.

Author information

  • 1CURE: Digestive Diseases Research Center VA GLAHS, Los Angeles, CA, USA; Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
  • 2Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA.
  • 3Division of Immunology, Beckman Research Institute of City of Hope, Duarte, CA, USA.
  • 4Department of Physiology, Tokushima Bunri University, Tokushima, Japan.
  • 5Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA. Electronic address: sayeghai@mytu.tuskegee.edu.

Abstract

Microisolation techniques utilizing several reverse phase high performance liquid chromatography (HPLC) steps have resulted in the purification of two rat gastrin releasing peptide (GRP) forms suitable for microsequence and mass spectral analysis. The sequence of the larger form is APVSTGAGGGTVLAKMYPRGSHWAVGHLM-amide and the smaller form is GSHWAVGHLM-amide which is the carboxyl terminal decapeptide of the larger peptide. The peptides were synthesized and their feeding patterns e.g. first meal size (MS), intermeal interval (IMI) and satiety ratio (SR, IMI/MS) were determined in overnight food-, but not water deprived, male Sprague Dawley rats. The peptides were administered in the femoral vein (0, 0.21, 0.41 and 1.03 nmol/kg) immediately before presenting the rats with a 10% sucrose solution. We found that (1) GRP-10 (all doses) and GRP-29 (0.41 nmol/kg) reduced first MS, (2) both peptides prolonged IMI length and (3) both peptides increased the SR to similar extents. In conclusion, GRP-10 and GRP-29 are the two endogenous forms of GRP in the rat intestine and they reduce short term feeding to similar extents when administered intravenously.

Published by Elsevier Inc.

KEYWORDS:

Gastrin releasing peptide-29; Intermeal interval; Intravenous; Meal size; Rat

PMID:
24993846
[PubMed - indexed for MEDLINE]
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