Stepwise splitting of ribosomal proteins from yeast ribosomes by LiCl

PLoS One. 2014 Jul 3;9(7):e101561. doi: 10.1371/journal.pone.0101561. eCollection 2014.

Abstract

Structural studies have revealed that the core of the ribosome structure is conserved among ribosomes of all kingdoms. Kingdom-specific ribosomal proteins (r-proteins) are located in peripheral parts of the ribosome. In this work, the interactions between rRNA and r-proteins of eukaryote Saccharomyces cerevisiae ribosome were investigated applying LiCl induced splitting and quantitative mass spectrometry. R-proteins were divided into four groups according to their binding properties to the rRNA. Most yeast r-proteins are removed from rRNA by 0.5-1 M LiCl. Eukaryote-specific r-proteins are among the first to dissociate. The majority of the strong binders are known to be required for the early ribosome assembly events. As compared to the bacterial ribosome, yeast r-proteins are dissociated from rRNA at lower ionic strength. Our results demonstrate that the nature of protein-RNA interactions in the ribosome is not conserved between different kingdoms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Isotope Labeling
  • Lithium Chloride / chemistry
  • Lithium Chloride / toxicity*
  • Peptides / analysis
  • Peptides / isolation & purification
  • Protein Structure, Quaternary
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / metabolism
  • Ribosomal Proteins / chemistry
  • Ribosomal Proteins / metabolism*
  • Ribosomes / drug effects
  • Ribosomes / metabolism
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / metabolism
  • Tandem Mass Spectrometry

Substances

  • Peptides
  • RNA, Ribosomal
  • Ribosomal Proteins
  • Lithium Chloride

Grants and funding

This work was supported by Estonian Research Foundation Grant Nr. 9289 and Estonian Research Council Institutional Research Grant Nr. 14021 (URL: www.etag.ee/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.