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J Anal Toxicol. 2014 Nov-Dec;38(9):681-5. doi: 10.1093/jat/bku081. Epub 2014 Jul 2.

Comparison of accumulation of clenbuterol and salbutamol residues in animal internal tissues, non-pigmented eyes and hair.

Author information

  • 1Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, Zagreb 10000, Croatia pleadin@veinst.hr.
  • 2Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, Zagreb 10000, Croatia.
  • 3Laboratory for Analyses of Veterinary Medicinal Products, Croatian Veterinary Institute, Savska 143, Zagreb 10000, Croatia.
  • 4Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb 10000, Croatia.

Abstract

The aim of the study was to compare the accumulation of β-adrenergic agonist residues clenbuterol (CLB) and salbutamol (SAL) in internal tissues, non-pigmented eyes and hair of laboratory animals repeatedly administered with CLB and SAL during 7 days. Experimental albino guinea pigs (n = 20) were treated with CLB (n = 10) and SAL (n = 10) in anabolic doses of 0.25 and 2.5 mg/kg, whereas the control animal group (n = 10) was left untreated. Methodology validation showed that the ELISA assay to be suitable for β-agonists' semiquantitative determination. The results revealed a significantly higher (P < 0.05) accumulation potential of CLB in comparison with SAL in all investigated tissues. Despite of their lack of pigmentation and the applied dose, the highest residual CLB concentrations were determined in the eyes of the studied animals, followed by their hair, liver, lungs, kidney, heart and adipose and muscle tissue, whereas residual SAL concentrations found in the eyes and hair of the administered animals did not significantly differ (P > 0.05) from those obtained in their internal tissues.

© The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

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