Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Dev Biol. 2014 Sep 1;393(1):71-83. doi: 10.1016/j.ydbio.2014.06.019. Epub 2014 Jun 28.

Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development.

Author information

  • 1Medical College of Wisconsin, Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, CRI C3420, 8701 Watertown Plank Road, P.O. Box 26509, Milwaukee, WI 53226, USA.
  • 2Division of Nephrology, Hypertension and Renal Transplantation, Room CG-98, 1600 Archer Road, University of Florida, Gainesville, FL 32610, USA.
  • 3Medical College of Wisconsin, Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, CRI C3420, 8701 Watertown Plank Road, P.O. Box 26509, Milwaukee, WI 53226, USA; Division of Nephrology, Hypertension and Renal Transplantation, Room CG-98, 1600 Archer Road, University of Florida, Gainesville, FL 32610, USA.
  • 4Medical College of Wisconsin, Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, CRI C3420, 8701 Watertown Plank Road, P.O. Box 26509, Milwaukee, WI 53226, USA. Electronic address: rramchan@mcw.edu.

Abstract

E-twenty six variant 2 (Etv2) transcription factor participates in cardiac, vascular-endothelial and blood cell lineage specification decisions during embryonic development. Previous studies have identified genomic elements in the etv2 locus responsible for vascular endothelial cell specification. Using transgenic analysis in zebrafish, we report here an etv2 proximal promoter fragment that prevents transgene misexpression in myocardial progenitor cells. This inhibition of etv2 expression in the cardiac progenitor population is partly mediated by Scl and Nkx2.5, likely through direct binding to the etv2 promoter, and cis-regulatory elements located in the first and second introns. The results identify an etv2 cis-regulatory mechanism controlling cardiovascular fate choice implying that etv2 participates in a transcriptional network mediating developmental plasticity of endothelial progenitor cells during embryonic development.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Cardiac disc; Etv2; Scl; Transcription; Zebrafish

PMID:
24984259
[PubMed - in process]
PMCID:
PMC4137469
[Available on 2015/9/1]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk